Differentially Expressed Genes in Cadmium-Exposed Sebastes Schlegeli Using Dd-Pcr

Received:1 March, 2014 Accepted: 12 April, 2014 Published: 20 May, 2014 The differentially expressed genes by exposure to environmental pollutants can provide the important information about the reaction mechanisms in the organism as well as adaptation to exterior stresses or environmental changes. Also they can be developed as biomarkers for the detection of environmental stress and exterior stimuli. Differential display polymerase chain reaction (DD-PCR) technique has been usefully used to explore the clones which expressions increased or decreased by exposure to environmental pollutants and this study aimed to search for those genes in cadmium-exposed rockfish (Sebastes schlegeli) using DD-PCR. The RNA isolated from liver of 100 μg/L Cd-exposed rockfish was used for screening of the differentially displayed genes and total 20 differentially expressed genes (DEG) are detected. The nucleotide sequences were analyzed. The present data provided the general information about the effect of cadmium contamination on marine organism and further more the primary step in development of new biomarkers for heavy metal pollution or environmental stresses. © 2014 PSCI Publisher All rights reserved.